Greetings sir, During an Intercourse the protection got torn, I came to know about it only after the act was over, I did not ejaculate, following this i took hiv rna pcr test with limit of detection 90 copies/ml on 15th and 33rd day after exposure, 4th gen ag/ab test on 16th, 33rd, 45th and 93rd day after exposure, the call girl i was with also took ab test from veinous blood on rapid card at local clinic, all the test mentioned were negative, is there any need to retest after 6 months? The girl has further agreed to take 4th gen test

Welcome to the forum. Thanks for your confidence in us.---

Thank you for the reply sir, does co infection of hepatitis prolong the window period, and transit of blood samples affect the accuracy, and after 100 days I feel a slight pain behind my ears am I late seroconverting?

Sir just to add to the above information i had taken rna pcr on 33rd day too

No, hepatitis does not significantly prolong the time to positive HIV testing. Pain behind ears doesn't go along with HIV seroconversion at all, and it never takes more than 6 weeks for the AgAb (4th generation) test to become positive, and never longer than 11 days for the RNA PCR. Your second RNA PCR test was a waste of money. Stop testing entirely and stop worrying! It is impossible you have HIV.---
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Thank you for the reply sir, does the accuracy apply even if I were immunocompromised, not that I am but would want to be 100% sure, as I am planning to get married soon and i don't want to destroy someone's life

This is a very common question and the answer is no; immunodeficiency of any kind does not alter the timing or reliability of any HIV tests. Concern about the possibility is common because the earliest HIV antibody-only tests might have had delays or missed infection from immune deficiency. But there is no such problem with the modern (3rd generation) antibody tests. Most important, the AgAb (4th generation) and HIV RNA PCR tests direct the virus itself -- and any effect from immune deficiency would be to make these positive even more strongly or earlier than otherwise. The bottom line is that even the most severe forms of immune deficiency and the most potent immune-suppressing drugs have no effect on reliability or timing of conclusive test results. No other medication has any such effect either, with the exception of anti-HIV drugs themselves:  if taken to prevent infection and it doesn't work, the time to positive test results is delayed.---