Went to London to a brothel type place in an apartment. Legality was dubious unsure if they are tested. 2 females perform oral sex while I had a condom on and wet wipe on pubic area. Was never comfortable so never got an erection. They asked if using a toy would help.I thought they meant on each other but I was laying on my back with eyes closed when I felt that they tried to place the toy in my anus.Felt pain/discomfort but dont think I was fully penetrated. Never saw any blood, didn't feel pain/soreness afterwards. Dildo had a condom on it. I felt violated and asked to leave. 1 asked if she could use oil to give me a handjob no condom. I agreed but after a few minutes I said stop, I washed my hands and left. Never had an erection. Panties never came off, my mouth never touched them and my hands never went in any orifices. I did pull one of their thongs to the side while she was on top of me and saw her anus/vagina with no visible lesions.

I have not had any symptoms at all. I took a 10 panel STD test with HIV 1 RNA early detection at Quest 13/14 days post exposure. Everything came back negative except iGG HSV1: 52.00 and HSV2 1.96.

1.Based on how early post exposure I tested for HSV can my results be from a previos exposure/false positives? Should I take a confirmatory hsv2 test from quest? I've never had any HSV symptoms

2.I read at 13/14 days HIV-1 RNA PCR negative test can be considered conclusive. Is this correct? Im taking it again along with a 4th gen at 28 days anyways.

3.Should i get any anal swab tests?

1.  Most SCWs in brothels do not have STIs.  Because of their vocation they tend to be careful and to get checked regularly.  As a  generalization, the risk of an encounter in the sort of brothel you describe is lower than the risk of a casual "hook up" in a bar.

2.  Receipt of oral sex is a no risk event for HIV (there has never been a case of HIV proven to have been acquired from receipt of orals sex) and very low risk for there STIs.

3. STIs are not transmitted through penetration of a dildo, particularly of it was cover by a condom.  

4.  Your tests prove you did not acquire an STI. 

In reply to your specific question:

No

I hope this information is helpful.  If anything I have said is unclear, please use your up to two follow-ups for clarification.   I would not be worried in the least.  EWH

Along those lines, I've put off any sexual activity with my wife in fear I may pass something on to her. When would it be safe to have unprotected sexual activity with her?

I also have an infant and toddler at home. Are they at any risks of transmitting anything from routine activities like diaper changes, showers/baths, etc? 

The RNA PCR test only tested for HIV-1, should I be concerned/test for HIV-2? 

I'm glad to hear the dildo is a low risk event as this was what had me the most concerned. I was worried that the condom could've not been intact or the dildo was not properly sanitized. Assuming either scenario was true, would that increase risk at all?

Thank you again for the service you're providing, it is truly appreciated.

STIs including HIV would not be transmitted by saliva, whether on a condom, on a dildo or due to oral contact with your anus.  Herpes is NOT spread in saliva but by DIRECT contact.  

I see no reason to abstain from unprotected sex with your wife.

Again, you are worrying entirely too much.  EWH

Rest assured, your responses have already been extremely beneficial in lowering my anxiety and I am feeling ready to just move past this event and move on with my life.

With that being said, I just want to repeat this question as you did not directly address it in your response:

I just want to clarify. Despite my tests being done only 13/14 days post exposure you do not believe it is warranted to test again for other STDs? I've read the window period for some STDs being in the 4-6-12 week range which is why I planned on testing again. 

I realize this will be your last response so I just want to take this final opportunity to thank you once again for your response and reassurance.

This completes this thread.  Take care.  EWH