Hoping you can help ease some anxieties I’ve been dealing with over this whole ordeal. 27 days ago I had protected vaginal intercourse with a female ex girlfriend. She was on her period when we had sex but I am confident the condom did not break as I checked after removal. I am prone to health anxiety so I have been freaking out ever since. At 19 days post potential exposure I had a 4th gen antigen/antibody HIV 1/2 blood test done which came back negative. At 24 days I had an HIV-1 RNA blood test done which also came back negative. Can I put my mind at ease and believe that I don’t have this disease now that I have these results?

You were at little or no risk for HIV. Had you asked ahead of time, I would have said HIV testing wasn't necessary. Given your assumed uncertainty about your ex-GF's risk for HIV and other STIs, you were smart to use a condom. It made an already near zero risk even lower.

More detail:  Assuming your ex gf isn't a sex worker, injection drug user, or the sex partner of such a person, the chance she has HIV is near zero. And condoms are very effective prevention. Further, blood exposure makes no difference:  the amount of HIV in menstrual blood is no higher than in vaginal fluids, sex during menstruation doesn't raise the risk of infection even if she had been infected. Even before you were tested, I would judge your risk of HIV from this event to be under one chance in a billion. More important, your test results are 100% proof you were not infected.

Thanks again for all the info. This forum has been a blessing for my anxiety.

I'm glad my comments have been helpful. Thanks for the thanks.