I had a possible HIV/HCV exposure.

Tests performed:

• 8 months after exposure: HIV-1 RNA Viral Load, HIV Ag/Ab, TP-Ab — all negative

• 8 months + 1 week: HCV Ab, TP-Ab, HIV Ag/Ab — all negative

• 8 months + 3 weeks: HCV RNA, HIV Ag/Ab — all negative

About 2 hours before these tests, I took 150 mcg of biotin (D-Biotin) from a multivitamin (https://iherb.co/KGWboLqB). I read that biotin can sometimes interfere with immunoassays.

My questions are:

1. Could this biotin affect RNA PCR results, or only Ag/Ab tests?

2. Are my results conclusive for ruling out HIV, HCV, and syphilis, or do I need any further testing?

Thank you very much for your help.