[Question #3255] Reading

37 months ago

Hello,


Thank you providing such a platform.


I needed some help in understanding the results


 i've done three tests (antibody/antigen)at a gap of approx 4 weeks between each


i understand its important to focus on the non reactive part, however is it ok if all three tests had an exact same result of lets say 0.21


 is this ok and normal to be the same ?  or would you recommend to test elsewhere again


the units were COI ; methodology was ECLIA

Edward W. Hook M.D.
Edward W. Hook M.D.
37 months ago
Welcome to our Forum.  I will be happy to comment.  Your negative HIV antibody tests should be beloved.  Low test values such as you have mentioned mean that there are no antibodies to HIV in your blood and such results on tests taken any time more that 8 weeks after an exposure indicate that you were not infected.  I see no reason for further testing related to past encounters at this time. EWH---
37 months ago
Thank you for your reply. Just some follow up if that's ok

1) My concern was all the three tests having an exact same value. 0.21
is this normal and ok to ignore

2) is eclia method an old dated ? you mention antibody test in the response but i was under the impression that the test i took looked for both antibody and antigen since the same was mentioned in the reading (1&2 Abs and P24Ag - serum)
Is the same as duo and the more reliable combo test or did i end up taking the old dated test
Edward W. Hook M.D.
Edward W. Hook M.D.
37 months ago
As I said above, when values are very low, they are reported out with the same numbers such as you have experienced.  This should not make you doubt the test result.  Believe the tests.

The ECLIA method uses luminescence to detect antibodies to HIV and performs similarly to EIA or ELISA tests.  Each of these tests tests only for HIV antibodies and are considered 3rd generation, antibody tests.  4th generation tests test both for antibodies and the HIV antigen and give definitive results a few weeks sooner than tests for antibodies but when performed at the right time, are equally good.  3rd generation tests give definitive results 8 weeks after an exposure while 4th generation tests give conclusive results 6 weeks after exposure.  As long as your tests were performed more than 8 weeks after your exposure of concern, your results are reliable and should be believed.  EWH
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37 months ago
Thanks again for your reply, i am clear on the first part, however unclear on the second.

Reason being the report reads out as below format:

Test/Parameters - HIV (1&2 Abs and P24Ag) serum

Units - COI
Ref Range - 
Methodology - ECLIA


Since it mention 1&2 abs and p24 ag ; i had been all along thinking i am doing the combo test only.. am i wrong ?

There is an other lab which has the same format as above but methodology is CLIA and units is index .. is this any different?

i apologize for any ignorance on my part, however i was not able to understand why the parameters would mention both when infact they test for only antibody

thank you and sorry
Edward W. Hook M.D.
Edward W. Hook M.D.
37 months ago
Thanks for the clarification.  Terms like ECLIA, EIA, ELISA all stand for the chemical method used in the test.  For instance, ECLIA stands for ElectroChemiLuminescence ImmunoAssay.   The chemical method used for your test should not matter to you as long as the test has been approved for use by an official body such as the U.S. FDA or the WHO and I suspect that is the case for your test if it was done in a reputable lab.   Most ELCIA assays test only for antibody but if the test mentions detection of both antibodies ("abs") and antigen ("ag"),  then it is a combination or 4th generation test.  None of this however changes my message to you- your test is negative, you did not get HIV from the exposure you are concerned about.  You need to believe the test and not worry about the test method. 

This is my third response to your questions.  I will leave the thread open for a few hours and then close it.  EWH
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37 months ago
thank you once again for the technicality of this and noted about closing the thread

i understand that even a eclia test can be a combo test

appreciated thanks

Edward W. Hook M.D.
Edward W. Hook M.D.
37 months ago
Correct.  Your results prove you do not have HIV form the encounter you have described.  EWH
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